How to collect a sample

Instructions for Canine DNA Sampling

DNA can be isolated from several types of canine biological material. Our laboratory accepts blood samples and cheek (buccal) swabs, which both provide high-quality DNA when collected correctly.

The most reliable source of high-quality DNA is a peripheral blood sample, which must be collected by a licensed veterinarian. Buccal swabs may be collected by the dog owner, following the instructions provided. To ensure a successful DNA extraction, swabbing must be carried out thoroughly and according to the recommended procedure.

Sampling kits are available for both blood collection and cheek swabs. If DNA cannot be isolated due to an insufficient sample, transport damage or any other technical issue, a replacement sampling kit will be provided free of charge.

Why is identity confirmation important?

Confirming the identity of the dog by an independent professional (such as a veterinarian) ensures the reliability and credibility of the test results. This confirmation is included in the final laboratory report.

Each sample must be accompanied by a completed sample collection form, containing:

- the dog’s full name

- breed

- microchip number or tattoo

- the name, signature and stamp of the veterinarian performing the sample collection (when applicable)

Most often, identity is verified using a microchip scanner or, where applicable, a tattoo. Some Kennel Clubs may have specific requirements, and certain organisations will only accept samples that were collected and confirmed by an authorised person.

Instructions for Blood Sampling

Blood sampling must always be performed by a licensed veterinarian.

Once your order has been completed, a Blood Sampling Kit will be sent to you.

The kit includes:

  • a sterile EDTA tube
  • a sealable bag with a sample label
  • a postal return envelope with the laboratory address

A blood sample may also be collected during a regular appointment with your veterinarian, even if you have not yet received a kit from us. In this case, please inform your veterinarian of the required sample volume and the type of tube needed (EDTA tube – violet top).

Test-tube (violet)

Blood Sampling Procedure

1. Use a new sterile syringe and needle for each dog.

2. Collect unclotted blood into the EDTA tube. A volume of 0.5–1 ml is sufficient for successful analysis.

3. Gently invert the tube several times to ensure the blood mixes thoroughly with the anticoagulant.

4. Place the tube into the labelled sample bag and seal it securely.

5. Clearly write the sample identification on the label (include the sample number if available).

6. Store the tube in the refrigerator (do not freeze) and send it to us as soon as possible.

7. When packing the sample, ensure the tube is protected from breaking or leaking during transport (e.g., by wrapping it in tissue or bubble wrap).

8. Samples should be sent by regular mail to:

Perro Genetics Limited
HQ Atlantic House
18–22 Hamilton Street
Birkenhead
CH41 1AL
United Kingdom (UK)

Instructions for Cheek Swab Sampling

Cheek swab sampling may be performed by the dog owner or a veterinarian. A Cheek Swab Sampling Kit will be sent to you once your order has been completed.

The kit includes:

- 2 sterile swabs

- a small envelope for the swabs

- a postal return envelope with the laboratory address

Cytobrush

Cheek Swab Sampling Procedure

1. Make sure the dog’s mouth is clean. Do not give food or water for 15 minutes before sampling.

2. Write the sample identification clearly on the small envelope (include the sample number if available).

3. Follow the steps below (A–D) with both swabs:

A) Open the swab package carefully. Avoid touching any surface other than the inside of the dog’s cheek.

B) Place the swab between the cheek and gum, and rotate it firmly for at least 5–10 seconds. Change direction during the movement to collect as many cells as possible. A visible amount of material should be present on the swab.

C) Allow the swab to air-dry for about one minute.

D) Insert the dried swab into the small envelope.

4. Seal the small envelope with both swabs inside.

5. Place the small envelope into the addressed return envelope and send it as soon as possible.

Send samples to:

Perro Genetics Limited
HQ Atlantic House
18–22 Hamilton Street
Birkenhead
CH41 1AL
United Kingdom (UK)

Sampling Nursing Puppies

To avoid contamination with the mother’s cells:

Wait 30 minutes after nursing before sampling.

Separate each puppy from the mother and littermates for 20 minutes to prevent mutual licking.

Offer normal food, then wait 20 minutes before sampling (no food or water during this time).